bhk 21 cell monolayers Search Results


bhk 21 monolayers  (ATCC)
99
ATCC bhk 21 monolayers
Bhk 21 Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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co 2  (World Precision Instruments)
93
World Precision Instruments co 2
Co 2, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ethylene-based polymers elitetm  (Dow Chemical)
90
Dow Chemical ethylene-based polymers elitetm
Ethylene Based Polymers Elitetm, supplied by Dow Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hela 229 cell monolayers  (ATCC)
94
ATCC hela 229 cell monolayers
In situ identification of C. pneumoniae in <t>HeLa</t> <t>229</t> cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.
Hela 229 Cell Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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promocell cell growth medium  (PromoCell)
97
PromoCell promocell cell growth medium
In situ identification of C. pneumoniae in <t>HeLa</t> <t>229</t> cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.
Promocell Cell Growth Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polytetrafluoroethylene filters transwell transwell® with pore polyester membrane insert  (Corning Life Sciences)
90
Corning Life Sciences polytetrafluoroethylene filters transwell transwell® with pore polyester membrane insert
In situ identification of C. pneumoniae in <t>HeLa</t> <t>229</t> cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.
Polytetrafluoroethylene Filters Transwell Transwell® With Pore Polyester Membrane Insert, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reagent protein expression sa g sf 21 cells recombinant monolayer bacmid dna  (Thermo Fisher)
99
Thermo Fisher reagent protein expression sa g sf 21 cells recombinant monolayer bacmid dna
In situ identification of C. pneumoniae in <t>HeLa</t> <t>229</t> cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.
Reagent Protein Expression Sa G Sf 21 Cells Recombinant Monolayer Bacmid Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sf9 cells  (ATCC)
99
ATCC sf9 cells
In situ identification of C. pneumoniae in <t>HeLa</t> <t>229</t> cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.
Sf9 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellbind flasks corning  (Corning Life Sciences)
90
Corning Life Sciences cellbind flasks corning
In situ identification of C. pneumoniae in <t>HeLa</t> <t>229</t> cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.
Cellbind Flasks Corning, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glass-bottom dishes mattek cat# p35g-1.5-21-c  (MatTek)
90
MatTek glass-bottom dishes mattek cat# p35g-1.5-21-c
In situ identification of C. pneumoniae in <t>HeLa</t> <t>229</t> cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.
Glass Bottom Dishes Mattek Cat# P35g 1.5 21 C, supplied by MatTek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alizarin red-s  (Millipore)
90
Millipore alizarin red-s
In situ identification of C. pneumoniae in <t>HeLa</t> <t>229</t> cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.
Alizarin Red S, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


In situ identification of C. pneumoniae in HeLa 229 cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.

Journal:

Article Title: Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization

doi: 10.1128/AEM.68.8.4081-4089.2002

Figure Lengend Snippet: In situ identification of C. pneumoniae in HeLa 229 cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.

Article Snippet: Chlamydial strains were maintained on cycloheximide-treated HeLa 229 cell monolayers (CCL2.1; American Type Culture Collection) in six-well culture plates by standard procedures.

Techniques: In Situ, Labeling, Fluorescence

Coinfection of HeLa 229 cells with C. trachomatis and C. pneumoniae. The simultaneous application of FLUOS-labeled probe Ct-623 specific for C. trachomatis (A) (green) and Cy5-labeled probe Cpn-214 specific for C. pneumoniae (B) (blue) allows the differentiation between the two chlamydia species. The simultaneous use of the Cy3-labeled eukaryotic probe EUK516 (C) (red) clearly demonstrated that a single HeLa 229 cell can be infected with both chlamydiae (D). Bar, 10 μm.

Journal:

Article Title: Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization

doi: 10.1128/AEM.68.8.4081-4089.2002

Figure Lengend Snippet: Coinfection of HeLa 229 cells with C. trachomatis and C. pneumoniae. The simultaneous application of FLUOS-labeled probe Ct-623 specific for C. trachomatis (A) (green) and Cy5-labeled probe Cpn-214 specific for C. pneumoniae (B) (blue) allows the differentiation between the two chlamydia species. The simultaneous use of the Cy3-labeled eukaryotic probe EUK516 (C) (red) clearly demonstrated that a single HeLa 229 cell can be infected with both chlamydiae (D). Bar, 10 μm.

Article Snippet: Chlamydial strains were maintained on cycloheximide-treated HeLa 229 cell monolayers (CCL2.1; American Type Culture Collection) in six-well culture plates by standard procedures.

Techniques: Labeling, Infection

Combination of FISH and DFA. The performance of DFA subsequent to FISH allows the identification of chlamydiae (by the rRNA-targeted oligonucleotide probes) simultaneously with the detection of chlamydial antigen (by antichlamydia antibodies). (A) Fluorescent signal derived from DFA using fluorescein-labeled antichlamydia-LPS antibodies. (B) Fluorescent signal derived from FISH with C. pneumoniae-specific probe Cpn-214 labeled with Cy3. (C) Fluorescent signal derived from DAPI staining of chlamydiae and nuclei of the HeLa 229 host cells. (D) Composite image. Bar, 10 μm.

Journal:

Article Title: Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization

doi: 10.1128/AEM.68.8.4081-4089.2002

Figure Lengend Snippet: Combination of FISH and DFA. The performance of DFA subsequent to FISH allows the identification of chlamydiae (by the rRNA-targeted oligonucleotide probes) simultaneously with the detection of chlamydial antigen (by antichlamydia antibodies). (A) Fluorescent signal derived from DFA using fluorescein-labeled antichlamydia-LPS antibodies. (B) Fluorescent signal derived from FISH with C. pneumoniae-specific probe Cpn-214 labeled with Cy3. (C) Fluorescent signal derived from DAPI staining of chlamydiae and nuclei of the HeLa 229 host cells. (D) Composite image. Bar, 10 μm.

Article Snippet: Chlamydial strains were maintained on cycloheximide-treated HeLa 229 cell monolayers (CCL2.1; American Type Culture Collection) in six-well culture plates by standard procedures.

Techniques: Derivative Assay, Labeling, Staining